Related Papers
Frontiers in Genetics
Correlation between the genotoxicity endpoints measured by two different genotoxicity assays: comet assay and CBMN assay
2015 •
Carina Ladeira
Biomarker
Genotoxicity Biomarkers: Application in Histopathology Laboratories
2012 •
Carina Ladeira, Elisabete Carolino, Susana Viegas
Mutation Research/Reviews in Mutation Research
Commentary: Critical questions, misconceptions and a road map for improving the use of the lymphocyte cytokinesis-block micronucleus assay for in vivo biomonitoring of human exposure to genotoxic chemicals—A HUMN project perspective
2014 •
Nina Holland
Nutrition and Metabolic Insights
Role of Macronutrients and Micronutrients in DNA Damage: Results From a Food Frequency Questionnaire
2017 •
Elisabete Carolino
AIMS Genetics
The use of genotoxicity biomarkers in molecular epidemiology: applications in environmental, occupational and dietary studies
2017 •
Carina Ladeira
Molecular epidemiology is an approach increasingly used in the establishment of associations between exposure to hazardous substances and development of disease, including the possible modulation by genetic susceptibility factors. Environmental chemicals and contaminants from anthropogenic pollution of air, water and soil, but also originating specifically in occupational contexts, are potential sources of risk of development of disease. Also, diet presents an important role in this process, with some well characterized associations existing between nutrition and some types of cancer. Genotoxicity biomarkers allow the detection of early effects that result from the interaction between the individual and the environment; they are therefore important tools in cancer epidemiology and are extensively used in human biomonitoring studies. This work intends to give an overview of the potential for genotoxic effects assessment, specifically with the cytokinesis blocked micronucleus assay an...
Journal of Hazardous Materials
Genotoxic biomonitoring of tobacco farmers: Biomarkers of exposure, of early biological effects and of susceptibility
2012 •
Mariangela Allgayer
Oxidative medicine and cellular longevity
Occupational Exposure to Pesticides in Tobacco Fields: The Integrated Evaluation of Nutritional Intake and Susceptibility on Genomic and Epigenetic Instability
2018 •
Juliana da Silva
Pesticides used at tobacco fields are associated with genomic instability, which is proposed to be sensitive to nutritional intake and may also induce epigenetic changes. We evaluated the effect of dietary intake and genetic susceptibility polymorphisms in (rs1801133) and (rs2736100) genes on genomic and epigenetic instability in tobacco farmers. Farmers, when compared to a nonexposed group, showed increased levels of different parameters of DNA damage (micronuclei, nucleoplasmic bridges, and nuclear buds), evaluated by cytokinesis-block micronucleus cytome assay. Telomere length (TL) measured by quantitative PCR was shorter in exposed individuals. Global DNA methylation was significantly decreased in tobacco farmers. The exposed group had lower dietary intake of fiber, but an increase in cholesterol; vitamins such as B, B, and C; -carotene; and -retinol. Several trace and ultratrace elements were found higher in farmers than in nonfarmers. The genotype influenced nucleoplasmic brid...
Mutation Research/Genetic Toxicology and Environmental Mutagenesis
Follow-up study of genotoxic effects in individuals exposed to oil from the tanker Prestige, seven years after the accident
2014 •
Francisco Aguilera
British journal of cancer
Increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of bladder cancer
2016 •
Rossana Critelli
Bladder cancer (BC) is among the most common malignancies worldwide. The identification of new biomarkers for early BC detection, recurrence/progression is urgently needed. The cytokinesis-block micronucleus assay (CBMN) evaluates chromosome damage in cultured human lymphocytes and micronuclei (MN) provide a convenient and reliable index of both chromosome breakage and loss. Chromosomal damage (expressed as frequencies of MN, nucleoplasmic bridges and nuclear buds (NBUD)) was evaluated by CBMN assay in cryopreserved lymphocytes from 158 age/smoking-matched pairs of cases and controls in relation to BC risk, recurrence or progression. Moreover, non-muscle invasive BC (NMIBC) patients were characterised for 783 DNA repair gene polymorphisms for their possible association with the investigated cytogenetic end points. MN and NBUD frequencies were significantly higher in cases than in controls (P=0.001 and P=0.006, respectively), with the associations being stronger in NMIBC. In a logistic regression model, for each increase of one unit in the MN frequency, a 1.12 increased risk of developing NMIBC was observed. In NMIBC cases, 10 polymorphisms were associated with different MN frequencies after genotype stratification. A model including traditional BC risk factors, MN frequency and the selected polymorphisms differentially distributed in cases and controls improved BC patient identification. Understanding the meaning of systemic chromosomal damage in BC patients with respect to the general population may help to adopt specific prevention strategies and therapeutic intervention.
Mutation Research-genetic Toxicology and Environmental Mutagenesis
Genotoxicity biomarkers in occupational exposure to formaldehyde—The case of histopathology laboratories
2011 •
Elisabete Carolino, Susana Viegas
